Gram Positive And Negative Flow Chart
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1GRAM
Negative
Positive
Oxidase
Catalase
Negative
Positive
Negative
Positive
(Bacillus)
MacConkey
Bacillus
Bacillus
Coccus
α hemolytic β hemolytic γ hemolytic
Coccus
MacConkey
Glucose +
Optochin
Clusters
Enterococcus
β hemolytic
Negative
Positive
faecalis
Listeria
Salt tolerant
monocytogenes
Haemophilus
Lactose
Coagulase
Negative
Positive
Susceptible
Resistant
Bile Esculin +
influenzae
Motility
SIMS +
Vibrio cholera Pseudomonas
Streptococcus
Streptococcus
Negative
Positive
Factors V & X
Bacitracin
Negative
Positive
aeruginosa
pneumoniae
viridans
TCBS +
Sorbitol /
(yellowcolonies)
β hemolytic
Novobiacin
Dextrose +
HEK (H2S)
Maltose
Susceptible
Resistant
Staphylococcus
CAMP --
CAMP +
aureus
Susceptible
Resistant
Negative
Positive Negative
Positive
Negative
Positive
Streptococcus
Streptococcus
pyogenes
agalactiae
Coagulase
Staphylococcus
Shigella
Salmonella
EHEC
All others
Neisseria
Neisseria
Negative
saprophyticus
gonorrhoeae
meningitidis
Staphylococcus
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