Results - Enzymes - Helena High School printable pdf download

Results - Enzymes - Helena High School

RESULTS – ENZYMES – CATALASE EXPERIMENT

Name

Part 1: Normal Catalase Activity

1. Draw a picture of a substrate an enzyme reaction and the resulting product (s). Label the active site,

substrate and products.

2. What is the equation for the enzymatic reaction in this lab?

3. What is the enzyme being studied in this lab? ______________________What is the substrate?

______________________what are the products?

Table: Catalase activity at room temperature 2 H2O2

Hypothesis: If

then,

Title: Catalase Activity at Room Temperatur

Samples

Reaction Rate (0-5)

Tube 1: Liver with initial H202

Tube 2: Liquid onto a new piece of liver

Tube 1: new H2O2 onto original liver.

4. Observe the bubbles; what gas is being released?

Has it gotten warmer or colder?

_______________ Is the reaction endothermic or exothermic? _____________________

5. What liquid is in the test tube following the reaction? ______________________ What happen when you

added more liver to this liquid? _______________________Why? (RSQ)

6. Are enzymes reusable? How do you know?(RSQ)

Part 2: The Effect of Temperature on Catalase

1. Hypothesis: If temperature is related to enzyme activity, then

Experimental Design:

Independent Variable:

Dependent variable

Experimental Group

Control Group

Chart 2 – Effect of Temperature on Rate of Enzyme Activity

Rate of Enzyme Activity (0 – 5)

Temperature

100

C (boiled liver)

Room Temp. = ________

Results - Enzymes - Helena High School