Blood Laboratory Lab Report Template Page 3

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The presence or absence of the Rh factor on the surface of the red blood cells is also an
important component of blood type. People with the Rh factor have a positive blood type, and
people that lack the Rh factor have a negative blood type. An Rh-negative person will not form
anti-Rh antibodies unless he or she has been exposed to the Rh factor, usually either through a
bad blood transfusion or birth. If an Rh-negative person is mistakenly given Rh-positive blood,
it will stimulate the production of anti-Rh antibodies. A transfusion reaction (agglutination) will
not occur with this transfusion, because it takes time for the body to start making anti-Rh
antibodies. If this person was to receive a second transfusion of Rh-positive blood, however, the
antibodies in the recipient’s plasma will react with the antigens in the donated blood.
Agglutination will occur and may lead to death.
Blood Typing Procedure
1. Place three drops of blood into each of the three wells (marked A, B, and Rh) on your
plastic plate.
2. Place three drops of anti-A antibody into the well marked A.
3. Place three drops of anti-B antibody into the well marked B.
4. Place three drops of anti-Rh antibody into the well marked Rh.
5. Mix each sample for approximately 30 seconds with a toothpick.
6. Determine the blood type for your subject.
Subject :_________________________
Blood Type: __________
If agglutination occurs in a well, this means that the antibody that you added found something to
bind to. If you look at the first example below, adding the anti-A antibody did not cause
agglutination, so the A antigen is not present. Adding the anti-B antibody did not cause
agglutination, so the B antigen is not present. Adding the anti-Rh antibody did cause
agglutination, meaning that the Rh antigen is present. So, this person’s blood type is O+.

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