Enzymes In Mitochondria Chemical Reactions Lesson Plan Template
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3Cellular Respiration Lab
Observations, Interpretations, and Experimental Design
Each partner is responsible for their own write-up
DO NOT WRITE ON THIS LAB HANDOUT!! PLEASE RETURN
Enzymes in mitochondria
C 6 H 12 O 6 + O 2
CO 2 + H 2 O + ATP
→
(cellular energy)
(glucose)
(oxygen)
(carbon dioxide)
Chemical reactions used in determining rate of cellular respiration
PHTH
PHTH
Pink
CO 2 + H 2 O
!! H 2 CO 3
NaOH + H 2 O
!
→
!
!!
→
(Clear)
Acidic
Basic
I. Initial Observations:
Working with your partner, familiarize yourself with the equipment and the procedures
for using the phenolphthalein indicator (PHTH) with the sodium hydroxide (NaOH).
See procedures and equipment below for determining CO
production.
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MATERIALS
• A clock that measures seconds
• Phenolphthalein solution (PHTH)
• Two-250-milliliter flasks
• 0.6 percent NaOH in a dropping bottle
• Water
(6 grams NaOH/1 liter)
• Soda straw
• A white sheet of paper
Measuring CO
production:
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Fill two 250-milliliter flasks with 150 ml of tap water. Add three drops of
phenolphthalein to each flask. Add sodium hydroxide (NaOH) to the water drop by
drop until you obtain a light pink color in both flasks; label ONE flask “control”. Label
the second flask “experimental”. Insert a straw into the experimental flask, and blow
gently into the water for 1 minute. (You may need to take one or two breaths; the total
exhalation time should be 1 minute.) Exhaled carbon dioxide dissolves in water to
create an acidic solution. (Water and carbon dioxide react to form carbonic acid.) As
the pH of the solution drops below 8, the PHTH in the solution loses its pink color.
Add NaOH to the experimental flask, drop by drop, and swirl gently to mix. Count
the number of drops it takes to restore the original pink color, comparing the color of
the experimental flask to the control flask. The total number of NaOH drops needed
to restore the original pink color will be your quantitative measurement of the amount
of CO
produced by you!
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Measuring Breathing rate:
Visually count the number of breaths taken in 30 seconds. Convert this to the number
of breaths per minute.
A. FIVE Observations:
(Label your paper with this subheading)
Working with your partner and using the equipment and procedures above, record five
good observations with respect to CO
production, breathing rate per minute.
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Make no interpretations!
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