Dna / Rna / Protein Synthesis / Ap Biology
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4Lab # _______
Name: __________________________
DNA / RNA / PROTEIN SYNTHESIS / AP Biology
Purpose:
To understand how DNA is replicated; How RNA is made from DNA and the process of how proteins are
synthesized (made) from DNA, mRNA, tRNA and rRNA.
Materials:
Scissors, construction paper, mRNA Codon Chart, Templates for bases, phosphates & sugars.
Procedure:
From each template, trace around it and cut out 12 of each; if you are lucky this was already done for you
by previous classes.
A = Adenine = Yellow
P = Phosphate
= Tan (cut 24)
C = Cytosine = Orange
DP =
= Black
DNA Polymerase
T = Thymine = Green
DL = DNA Ligase
= White
G = Guanine = Red
H = Helicase
= Brown
U = Uracil
= Light Blue
To = Topoisomerase = Dark Blue
D =
= Purple (cut 24)
DPr = DNA Primase = Black/White Striped
Deoxyribose
R = Ribose
= Pink
RP = RNA Primase
= Red / White Striped
Mark each with the proper letter on BOTH sides; With your newly cut nucleotides = (base, sugar &
phosphate) construct the following DNA and RNA molecules; transcribe the message into RNA when asked
to do so; and translate the message into amino acids (protein) when directed.
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Construct (lay out) the following DNA molecule on one side of your lab table; then find the matching
1)
letters (complement strand) of DNA bases and lay it out across from it. Be sure to also include the
“backbone” the „D‟ and „P‟ along both sides; and the enzymes involved with DNA Replication. WRITE
DOWN THE COMPLEMENT STRAND IN THE TABLE BELOW.
Remember to have it initialed before you
disassemble
DNA Parental Strand
Teachers Initials
a.
ACT
CGG
CAT
ACA
GGT
b.
TCT
TGA
CGG
GAA
TAG
c.
AAT
GCC
TAC
TTA
CCC
#1
DNA Complement Strand
a.
b.
c.
2)
RNA from DNA. Construct the following DNA molecule; then find the matching RNA letters; place them
on the opposite side – similar to #1 above but using RNA (remember you must have one side with DNA
(sugar= purple) and the other side with RNA (sugar = pink). Be sure to also include the “backbone” the
„D‟ and „P‟ along one side and „R‟ and „P‟ along the other side; and the enzymes involved with DNA to
RNA transcription.
WRITE DOWN THE COMPLEMENT STRAND OF RNA IN THE TABLE BELOW before
you disassemble it.
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