Science Lab Template Affects On Enzyme Reaction Rate (Part A)

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Affects on Enzyme Reaction Rate (Part A)
CLASS SET – PLEASE RETURN!
Background Information:
The compound hydrogen peroxide (H
O
), is a by-product of metabolic reactions in most living things (cells).
2
2
However, hydrogen peroxide is damaging to delicate molecules inside cells. As a result, nearly all organisms
contain the enzyme peroxidase, which breaks down H
O
as it is formed. Potatoes are one source of peroxidase.
2
2
Peroxidase speeds up the breakdown of hydrogen peroxide (H
O
) into water and gaseous oxygen. This reaction
2
2
can be detected by observing the oxygen bubbles generated. See reaction below.
H
O
+ peroxidase
H
O + O
2
2
2
2 gas
(Enzyme in potato)
(bubbles/foam)
Problem:
Does the enzyme peroxidase work in cold temperatures? Does peroxidase work better at higher
temperatures? Does peroxidase work after being frozen or boiled?
Materials provided:
For example, a clock or timer, 400-mL beaker, test tube holder, scalpel, beaker tongs, pieces of potato,
calibrated vials, 17% hydrogen peroxide(H
O
), ice, hotplate, and °C thermometer.
2
2
Plan the experiment:
• Decide on a way to test your hypothesis. Keep the available materials in mind.
• When testing the activity of the enzyme at a certain temperature, consider the length of time it will
take for the potato to reach that temperature, and how the temperature will be measured. Be sure to
completely cover the potato with hydrogen peroxide.
• To test for peroxidase activity, add several drops of hydrogen peroxide(H
O
) to the potato.
2
2
• When heating a piece of potato, first place it in a calibrated vial that is then placed in a small
amount of water in the beaker (a water bath). Then heat the beaker slowly so that the temperature
of the water and the temperature of the potato are always the same.
• Make observations at 4 different temperatures between 5 °C and 95 °C.
HINT: Room temperature is about 26 °C and does not require a water bath!
Check the plan: (discuss the following points with your partner to decide on your final procedure for
your experiment).
• What data will you collect (data tables).
• What factors or variables should be controlled?
• What temperatures will you test?
• How will you achieve those temperatures?
• How many trials will you run?
• How many trials will you run for each temperature?
Identify the variables:
• What is the independent variable (X) (the cause)?
• What is the dependent variable (Y) (the effect)?
• What factors do you need to control in your tests?
Please follow the order
of your coversheet and
Data:
check the box on cover
• Make a table including all data to be collected with proper units!
sheet when completed.
• Remember to run three trials in order to calculate averages.
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