Mutations Activity Biology Worksheets (Page 2 of 4) in pdf

Mutations Activity Biology Worksheets Page 2

Name: ______________________________________

TOC#_____

4. An insertion and deletion will cause a “frameshift.” Explain what this means. Is this more or less likely

to cause a noticeable mutation than a substitution?

5. Refresh your knowledge on how to use the universal codon chart below

AUG codes for:

CCG codes for:

GAU codes for:

UAA codes for

Procedures:

1. Using the DNA strand on the “Mutation Consequences Worksheet” transcribe the DNA into mRNA.

2. Using the Universal Codon Chart found in the pre-lab translate the mRNA into its corresponding amino

acid sequence.

3. Obtain a six pieces of paper labeled 1 through 6 from your teacher. Place them face down on your table.

4. The first nucleotide that will be mutated is the 4

nucleotide in the strand of DNA.

5. Each student will pick a slip of paper and complete the worksheet for the specific mutation they drew,

by following the instructions below.

6. No student in a group may use the same number.

Number

Corresponding Action

Drawn

Substitute specified nucleotide with an A

Substitute specified nucleotide with a C

Substitute specified nucleotide with a G

Substitute specified nucleotide with a T

Delete the nucleotide

Insert a nucleotide immediately after the specified nucleotide. Toss the die

again until you roll 1–4 to determine which nucleotide will be inserted.

7. Write the complete DNA sequence with one mutation in nucleotide 4.

8. Complete the mRNA sequence from the mutated DNA.

9. Translate the amino acid sequence from the mRNA.

10. Circle any differences from the original protein produced.

11. Repeat this activity again having the mutation occur at nucleotide 6. Each group member must use a

new mutation.

12. Answer the questions.

Mutations Activity Biology Worksheets Page 2