Entrance Test For Biotechnology Page 7
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23Part-‐-‐
B
31. The
n umber
o f
t imes
a
e ukaryotic
c ell
c an
u ndertake
r eplication
o f
i ts
D NA
p er
cell
c ycle
i s
a) Once,
i rrespective
o f
c ell
t ype
b) Infinite
f or
a
s tem
c ell
c) As
m any
a s
t he
n umber
o f
c hromosomes
i n
t he
c ell
d) Can
b e
a ltered
c hemically
b y
o ver
a ctivating
C DKs
32. DNA
f ragments
d uring
r eplication
a re
s ynthesized
w ith
t he
o rientation
a) 5’
à
3 ’
b) 3’
à
5 ’
c) 5’
à
3 ’
f or
l eading,
3 ’
à
5 ’
f or
l agging
s trands
d) 3’
à
5 ’
f or
l eading,
5 ’
à
3 ’
f or
l agging
s trands
33. If
the
Meselson-‐Stahl
experiment
were
to
be
performed
such
that
the
bacteria
growing
in
normal
media
are
introduced
to
heavy
isotope
media
before
cell
division,
t he
b anding
p attern
i n
F 3
g eneration
w ould
r esemble
m ost
a) 75%
N 14
–
2 5%
h ybrid
–
0 %
N 15
b) 44%
N 14
–
5 0%
h ybrid
–
6 %
N 15
c) 0%
N 14
–
2 5%
h ybrid
–
7 5%
N 15
d) 6%
N 14
–
5 0%
h ybrid
–
4 4%
N 15
34. The
c orrect
d efinition
o f
“ gene”
r efers
t o
a) Any
u nit
o f
c hromosome
w ith
a
s pecific
s equence
b) Sequence
o f
D NA
e ncoding
f or
p rimary
R NA
t ranscript
c) Sequence
o f
D NA
c orresponding
t o
m ature
m RNA
d) Sequence
o f
D NA
b etween
t ranslational
S TART
a nd
S TOP
s ites
35. In
order
to
identify
if
a
cell
is
undergoing
active
transcription,
one
could
use
radiolabelled
A TP
a nd
m easure
t he
a mount
o f
r adioactivity
i n
R NA
i solated
f rom
these
cells.
In
order
for
the
radioactivity
to
be
detected,
which
radiolabel
should
be
u sed?
a) [α-‐P
]
A TP
32
b) [β-‐P
]
A TP
32
c) [γ-‐P
]
A TP
32
d) [α-‐P
]
T TP
32
36. While
D NA
p olymerase
m isincorporates
n ucleotides
a t
t he
r ate
o f
1
i n
1 0
,
t he
5
actual
e rror
r ate
o bserved
i n
r eplicating
c ells
i s
1
i n
1 0
.
T his
i s
m ainly
a chieved
9
due
t o
a) Excision
o f
m isincorporated
n ucleotides
b) 3’
à
5 ’
p roofreading
a ctivity
o f
D NA
p olymerase
c) 5’
à
3 ’
p roofreading
a ctivity
o f
D NA
p olymerase
d) Replication
f ork
a rrest
7
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