Amino Acid Pka Chart Page 2
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1
2
3
4Titration of L-Aspartic Acid, an acidic amino acid (pKa
1.9, pKa
9.6; pKa
3.7)
1
2
R
HO
C
2
+
H
N
CO
H
3
2
L-Aspartic Acid
(fully protonated)
____ < pH < _____
____ < pH < _____
pH > _____
pH < _____
Isoelectric Point, pI =
Titration of L-Lysine, a basic amino acid (pKa
2.2; pKa
9.0; pKa
10.5 )
1
2
R
+
H
N
3
+
H
N
CO
H
3
2
L-Lysine
(fully protonated)
pH < _____
____ < pH < _____
____ < pH < _____
pH > _____
Isoelectric Point, pI =
Electrophoresis – separation of amino acids based on charge.
@ pH 5, Leu = ____; Asp = _____; Lys = _____
@ pH 9, Leu = ____; Asp = _____; Lys = _____
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