Cytochrome-C Comparisons Lab Page 2
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AMINO ACID SEQUENCES IN CYTOCHROME-C PROTEINS FROM DIFFERENT SPECIES
10
20
30
40
50
Amino Acid Number ----> 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9
Human, Chimpanzee.
- - - - - - - -
G
D V E K
G
K K I
F
I M K
C
S Q
C H
T V E K G G K H
K
T
G P
N
L
H
G
L F G
R
K T
G
Q A P
G
Y S
Y
T A
A
Rhesus monkey.....
- - - - - - - -
G
D V E K
G
K K I
F
I M K
C
S Q
C H
T V E K G G K H
K
T
G P
N
L
H
G
L F G
R
K T
G
Q A P
G
Y S
Y
T A
A
Horse ............
- - - - - - - -
G
D V E K
G
K K I
F
V Q K
C
A Q
C H
T V E K G G K H
K
T
G P
N
L
H
G
L F G
R
K T
G
Q A P
G
F T
Y
T D
A
Donkey ...........
- - - - - - - -
G
D V E K
G
K K I
F
V Q K
C
A Q
C H
T V E K G G K H
K
T
G P
N
L
H
G
L F G
R
K T
G
Q A P
G
F S
Y
T D
A
Common zebra .....
- - - - - - - -
G
D V E K
G
K K I
F
V Q K
C
A Q
C H
T V E K G G K H
K
T
G P
N
L
H
G
L F G
R
K T
G
Q A P
G
F S
Y
T D
A
Pig, Cow, Sheep...
- - - - - - - -
G
D V E K
G
K K I
F
V Q K
C
A Q
C H
T V E K G G K H
K
T
G P
N
L
H
G
L F G
R
K T
G
Q A P
G
F S
Y
T D
A
Dog...............
- - - - - - - -
G
D V E K
G
K K I
F
V Q K
C
A Q
C H
T V E K G G K H
K
T
G P
N
L
H
G
L F G
R
K T
G
Q A P
G
F S
Y
T D
A
Gray whale .......
- - - - - - - -
G
D V E K
G
K K I
F
V Q K
C
A Q
C H
T V E K G G K H
K
T
G P
N
L
H
G
L F G
R
K T
G
Q A V
G
F S
Y
T D
A
Rabbit............
- - - - - - - -
G
D V E K
G
K K I
F
V Q K
C
A Q
C H
T V E K G G K H
K
T
G P
N
L
H
G
L F G
R
K T
G
Q A V
G
F S
Y
T D
A
Kangaroo..........
- - - - - - - -
G
D V E K
G
K K I
F
V Q K
C
A Q
C H
T V E K G G K H
K
T
G P
N
L
N
G
I F G
R
K T
G
Q A P
G
F T
Y
T D
A
Chicken, Turkey...
- - - - - - - -
G
D I E K
G
K K I
F
V Q K
C
S Q
C H
T V E K G G K H
K
T
G P
N
L
H
G
L F G
R
K T
G
Q A E
G
F S
Y
T D
A
Penguin...........
- - - - - - - -
G
D I E K
G
K K I
F
V Q K
C
S O
C H
T V E K G G K H
K
T
G P
N
L
H
G
I F G
R
K T
G
Q A E
G
F S
Y
T D
A
Pekin duck........
- - - - - - - -
G
D V E K
G
K K I
F
V Q K
C
S Q
C H
T V E K G G K H
K
T
G P
N
L
H
G
L F G
R
K T
G
Q A E
G
F S
Y
T D
A
Snapping turtle...
- - - - - - - -
G
D V E K
G
K K I
F
V Q K
C
A Q
C H
T V E K G G K H
K
T
G P
N
L
N
G
L I G
R
K T
G
Q A E
G
F S
Y
T E
A
Rattlesnake.......
- - - - - - - -
G
D V E K
G
K K I
F
S M K
C
G T
C H
T V E E G G K H
K
T
G P
N
L
H
G
L F G
R
K T
G
Q A V
G
Y S
Y
T A
A
Bullfrog..........
- - - - - - - -
G
D V E K
G
K K I
F
V Q K
C
A Q
C H
T C E K G G K H
K
V
G P
N
L
Y
G
L I G
R
K T
G
Q A A
G
F S
Y
T D
A
Tuna..............
- - - - - - - -
G
D V A K
G
K K T
F
V Q K
C
A Q
C H
T V E N G G K H
K
V
G P
N
L
W
G
L F G
R
K T
G
Q A E
G
Y S
Y
T D
A
Screwworm fly.....
- - - - G V P A
G
D V E K
G
K K I
F
V Q R
C
A Q
C H
T V E A G G K H
K
V
G P
N
L
H
G
L F G
R
K T
G
Q A A
G
F A
Y
T N
A
Silkworm moth.....
- - - - G V P A
G
N A E N
G
K K I
F
V Q R
C
A Q
C H
T V E A G G K H
K
V
G P
N
L
H
G
F Y G
R
K T
G
Q A P
G
F S
Y
S N
A
Tomato horn “worm”
- - - - G V P A
G
N A D N
G
K K I
F
V Q R
C
A Q
C H
T V E A G G K H
K
V
G P
N
L
H
G
F F G
R
K T
G
Q A P
G
F S
Y
S N
A
Wheat ............
A S F S E A P P
G
N P D A
G
A K I
F
K T K
C
A Q
C H
T V D A G A G H
K
Q
G P
N
L
H
G
L F G
R
Q S
G
T T A
G
Y S
Y
S A
A
Rice .............
A S F S E A P P
G
N P K A
G
E K I
F
K T K
C
A Q
C H
T V D K G A G H
K
Q
G P
N
L
N
G
L F G
R
Q S
G
T T P
G
Y S
Y
S T
A
Baker's yeast (Fungus)
- - - T E F K A
G
S A K K
G
A T L
F
K T R
C
E L
C H
T V E K G G P H
K
V
G P
N
L
H
G
I F G
R
H S
G
Q A Q
G
Y S
Y
T D
A
Candida yeast(Fungus)
- - P A P F E 0
G
S A K K
G
A T L
F
K T R
C
A E
C H
T I E A G G P H
K
V
G P
N
L
H
G
I F S
R
H S
G
Q A Q
G
Y S
Y
T D
A
Neurospora (Fungus)
- - - - G F S A
G
D S K K
G
A N L
F
K T R
C
A E
C H
G E G G N L T Q
K
I
G P
A
L
H
G
L F G
R
K T
G
S V D
G
Y A
Y
T D
A
[CONTINUED FROM ABOVE]
60
70
80
90
100
110
Amino Acid Number-> 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2
Human, Chimpanzee.
N
K N K G I I
W
G E D T L M E
Y L
E
N P K K Y I P G T K M
I
F
V
G
I
K K
K E E
R
A
D
L I A Y L K K A T N E
AMINO ACID SYMBOLS
Rhesus monkey.....
N
K N K G I T
W
G E D T L M E
Y L
E
N P K K Y I P G T K M
I
F
V
G
I
K K
K E E
R
A
D
L I A Y L K K A T N E
A
= Alanine
Horse ............
N
K N K G I T
W
K E E T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K T E
R
E
D
L I A Y L K K A T N E
C
= Cysteine
Donkey ...........
N
K N K G I T
W
K E E T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K T E
R
E
D
L I A Y L K K A T N E
D
= Aspartic acid
Common zebra .....
N
K N K G I T
W
K E E T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K T E
R
E
D
L I A Y L K K A T N E
E
= Glutamic acid
Pig, Cow, Sheep...
N
K N K G I T
W
G E E T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K G E
R
E
D
L I A Y L K K A T N E
F
= Phenylalanine
Dog...............
N
K N K G I T
W
G E E T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
T G E
R
A
D
L I A Y L K K A T K E
G
= Glycine
Gray whale........
N
K N K G I T
W
G E E T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K G E
R
A
D
L I A Y L K K A T N E
H
= Histidine
Rabbit............
N
K N K G I T
W
G E D T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K D E
R
A
D
L I A Y L K K A T N E
I
= Isoleucine
Kangaroo..........
N
K N K G I I
W
G E D T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K G E
R
A
D
L I A Y L K K A T N E
K
= Lysine
Chicken, Turkey...
N
K N K G I T
W
G E D T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K S E
R
V
D
L I A Y L K D A T S K
L
= Leucine
Penguin...........
N
K N K G I T
W
G E D T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K S E
R
A
D
L I A Y L K D A T S K
M
= Methionine
Pekin duck........
N
K N K G I T
W
G E D T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K S E
R
A
D
L I A Y L K D A T A K
N
= Asparagine
Snapping turtle...
N
K N K G I T
W
G E E T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K A E
R
A
D
L I A Y L K D A T S K
P
= Proline
Rattlesnake.......
N
K N K G I I
W
G D D T L M E
Y L
E
N P K K Y I P G T K M
V
F
T
G
L
K K
K K E
R
T
D
L I A Y L K E A T A K
Q
= Glutamine
Bullfrog..........
N
K N K G I T
W
G E D T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K G E
R
Q
D
L I A Y L K S A C S K
R
= Arginine
Tuna..............
N
K S K G I V
W
N N D T L M E
Y L
E
N P K K Y I P G T K M
I
F
A
G
I
K K
K G E
R
Q
D
L V A Y L K S A T S -
S
= Serine
Screwworm fly.....
N
K A K G I T
W
Q D D T L F E
Y L
E
N P K K Y I P G T K M
I
F
A
G
L
K K
P N E
R
G
D
L I A Y L K S A T K -
T
= Threonine
Silkworm moth.....
N
K A K G I T
W
G D D T L F E
Y L
E
N P K K Y I P G T K M
V
F
A
G
L
K K
A N E
R
A
D
L I A Y L K E S T K -
V
= Valine
Tomato horn “worm”
N
K A K G I T
W
Q D D T L F E
Y L
E
N P K K Y I P G T K M
V
F
A
G
L
K K
A N E
R
A
D
L I A Y L K Q A T K
W
= Tryptophan
Wheat.............
N
K N K A V E
W
E E N T L Y D
Y L
L
N P K K Y I P G T K M
V
F
P
G
L
K K
P Q D
R
A
D
L I A Y L K K A T S S
Y
= Tyrosine
Rice .............
N
K N M A V I
W
E E N T L Y D
Y L
L
N P K K Y I P G T K M
V
F
P
G
L
K K
P Q E
R
A
D
L I S Y L K E A T S -
Baker's yeast.....
N
I K K N V L
W
D E N N M S E
Y L
T
N P K K Y I P G T K M
A
F
G
G
L
K K
E K D
R
N
D
L I T Y L K K A C E -
Candida yeast.....
N
K R A G V E
W
A E P T M S D
Y L
E
N P K K Y I P G T K M
A
F
G
G
L
K K
A K D
R
N
D
L V T Y M L E A S K -
Neurospora (Fungus)
N
K Q K G I T
W
D E N T L F E
Y L
E
N P K K Y I P G T K M
A
F
G
G
L
K K
D K D
R
N
D
I I T F M K E A T A -
Symbols in
light blue
or
gray
represent amino acids which show NO differences in any organism on the list, so you can ignore them.
(Adapted from Strahler, Arthur, Science & Earth History, 1987, p. 348)
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Cytochrome Clab pt 1
kew 04/02
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