Lab: Blood Exploration (Page 4 of 6) in pdf

Lab: Blood Exploration Page 4

PART B: Introduction to ABO Blood Typing

1. Place a piece of white paper (preferably with print) behind the blood typing plates.

2. On the paper behind the plates, label each plate as follows:

a. “#1” = Mr. Jones

b. “#2” = Mr. Smith

c. “#3” = Mr. Green

d. “#4” = Ms. Brown

3. Place 2-3 drops of simulated Mr. Jones’ blood into each well (“A”, “B”, and “Rh”) on plate #1.

4. Next, place 2 drops of simulated anti-A agglutinin serum in well “A”, 2 drops of simulated

anti-B agglutinin serum in well “B”, and 2 drops of simulated anti-Rh agglutinin serum in well

“Rh”.

5. Using the clean end of a toothpick thoroughly mix each well for approximately 30 seconds for

all the well plates. It is important to use a clean end of a toothpick for each well as you do not

want to cross-contaminate your samples!

6. Observe each well and record results in the data table. If you are uncertain as to whether or not

agglutination occurred, try reading a sample of text through the blood. If you cannot read the

text, agglutination did occur.

a. If agglutination occurred in a well, mark a “

” to indicate a positive reaction.

—

b. If no agglutination occurred, mark a “

“ to indicate a negative reaction.

7. Use the results of your agglutination tests to determine the ABO blood type of the individual

(A, B, AB, or O and Rh+ or Rh-).

8. Dispose of all blood samples in the sink. Thoroughly wash and dry the well plates and

toothpicks for reuse before returning your materials to the baggie.

Data

Table 1: Agglutination Results

ABO Blood

Anti-A Serum

Anti-B Serum

Anti-Rh serum

Blood Sample

(+ or –)

Type

#1-Mr. Jones

#2-Mr. Smith

#3-Mr. Green

#4-Ms. Brown

Lab: Blood Exploration Page 4