Gram Positive/negative Chart
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1
2Gram Positive
BACILLI
COCCI
Anaerobic
Aerobic
Anaerobic
Aerobic
Sporeformers:
Non-sporeformers:
Peptostreptococcus
Clostridium species
Propionibacterium
Eubacterium
Catalase test
Bifidobacterium
Actinomyces
(-)
(+)
Mobiluncus species
Streptococcus
Staphylococcus
Hemolytic Properties on Blood Agar
Coagulase test
(-)
(+)
Beta hemolysis
Alpha hemolysis
Gamma hemolysis
S. epidermidis
S. aureus
(complete; clear)
(partial; green)
(none)
S. saprophyticus
Bile esculin test (+)
S. hemolyticus
PYR test
Strep, Group D
(-)
(+)
Sporeformers:
Non-sporeformers:
Group A
Bacillus species
Listeria monocytogenes
CAMP test
Growth in 6.5% NaCl
S. pyogenes
Erysipelothrix
Or PYR test
(+)
(-)
Corynebacterium species
Optochin test &
Strep,
Lactobacillus species
Bile solubility test
Group B
(-)
(+)
Nocardia
Streptococcus, Group D
Enterococcus species
Latex agglutination test;
NOT Enterococcus
E. Faecium
Other Lancefield groups
(S. bovis)
E. Faecalis
C, F, G, & S. milleri group
(only speciated if
Vanco resistant)
(-) (-)
(+) (+)
S. viridans
S. pneumoniae
(misc. strep group)
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