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Cellulose Synthase Genes Where Do We Start

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Cellulose Synthase Genes: Where do we start?

Table 1: One of the conserved amino acid sequence of all cellulose synthase genes is DDG. Use the information in Figure

1 and write the full name for each of these amino acids.

1-letter abbreviation

Amino acid name

Table 2: Possible codons which could product the indicated amino acids.

Aspartic Acid

Glycine

List all possible codons for

these amino acids:

Table 3: Determining a DNA sequence that is complementary to the given piece of RNA which would code for the

production of the DDG portion of a CESA polypeptide.

Amino acid sequence

Aspartic Acid—Aspartic acid ---Glycine

One possible RNA sequence

GACGAUGGG

Complementary DNA sequence

Table 4: For any section of DNA sequence submitted to one of the databases, the position of the proper reading frame is

initially unknown. Until the sequence is analyzed, it is also unknown whether the sequence is from the sense or

antisense strand of the DNA molecule. You will analyze a small section to determine the proper reading frame and if it is

the sense or antisense strand of DNA. Follow the models. Use the codon chart in Figure 2 to determine the amino acids.

The following is a small sample sequence of nucleotides was submitted to a database:

GGCTGCTACCCT

In the spaces below, translate the six possible amino acid sequences for which this might code. Remember,

when reading the codon chart, substitute uracil where you see thymine:

Reading frame 1, as if this was the sense strand



GGC TGC TAC CCT

glycine – cysteine – tyrosine - proline

Reading frame 2, as if this was the sense strand



G GCT GCT ACC CT

Reading frame 3, as if this was the sense strand



GG CTG CTA CCC T

Cellulose Synthase Genes Where Do We Start