Dna/rna Genetics Worksheet With Answers Page 4

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a. The carboxyl end of AA #3 should now be covalently bonded directly to
(tRNA #2) (tRNA #3) (tRNA #4) (AA #2) (AA #3)
(AA
#4) (none of these).
It starts out attached to tRNA#3, but is transferred to AA#4 as soon as it arrives.
b. Which tRNA will you find in the A site? (tRNA #1) (tRNA #2) (tRNA #3)
(tRNA
#4) (tRNA #5).
The tRNA in the A site will carry (an amino acid)
(a
polypeptide) (neither)
c.
(either one).
Initially, the tRNA simply carries an amino acid, but then the growing
polypeptide is transferred to the A site before translocation. That peptide bond can
still be formed in the presence of erythromycin.
11. Here is a short stretch of replicating DNA from the bacterium E. coli. The top strand
is the template. Assume the ****** are the RNA primers.
This shows parts of four Okazaki fragments.
3’-------------------------------------------------------------------------------------5’
5’-----********--------------------*********-------------------*******----------------*3’
^
^
a|
b|
1
2
3
4
Which was made first, the Left or the Right fragment?
RIGHT
Which primer will be the first to be removed, Left, Middle, or Right?
RIGHT
When the primer in the middle is removed and filled in with DNA, the fragments must
then be joined.
Which enzyme removes the RNA primer?
DNA polymerase I
Which enzyme joins the fragments?
ligase
Where is the final connection made? (a or b?)
B

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