Tca Cycle Chemistry Paper Page 3
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2
3
4Answer Key
1. Biotin is used in ATP-dependent carboxylation reactions.
Lipoic acid participates in oxidation of hydroxy groups to carbonyls and forms a covalent
adduct with the substrate undergoing oxidation.
thiamin pyrophosphate- (1) decarboxylation of α−keto acids and (2) formation and cleavage
of α−hydroxy ketones
flavin adenine dinucleotide (FAD)- two electron donor and acceptor – 2 hydrogen donor
and acceptor- oxidation/reduction reactions
co-enzyme A- carrier of acyl units, formation of thioester linked acyl units
NAD- hydride donor and acceptor-participates in oxidation reduction reactions.
2. Use enzymes from TCA cycle:
O
O -
H
O
CH
C
3
2
O
+
C
O
CoA
S CoA
(C H
)
2
2
1
CO O -
acetyl CoA
α -ketoglutarate
O
O -
C
Homocitric acid
-O O C
H
C
C
O H
2
(C H
)
2
2
CO O -
1. Citrate synthase
2
2. Aconitase
+
+
NADH + H
NAD
O
O
O -
O -
C
C
3. Isocitrate dehydrogenase
C
O
-O O C
HC
C
H
CH
O H
(C H
)
2
2
2
3
C O
2
(C H
)
CO O -
2
2
CO O -
α -ketoadipic acid
3. Since succinate accumulates, this means that the conversion of succinate to fumarate is
blocked. Therefore the enzyme inhibited is succinate dehydrogenase.
- -
3
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