Vacuette Selection Chart

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VACUETTE
®
TUBE SELECTION CHART
CLSI Guidelines (6th ED). For more information consult Laboratory Handbook
Cap
Special
Cat No.
Cap Ring
Tube
Tests
Colour
Contents
Instructions
Draw Vol.
Colour
REQUIRED TESTS IN THE ORDER OF DRAW SHOWN
TAKE BLOOD CULTURES FIRST, THEN THE
HAEMATOLOGY REQUEST
Send to lab ASAP
*
KFK 343
Trisodium
• PT/APTT/Fibrinogen/D Dimer – 1
Tube*
• Thrombophilia Screen – 4
Tubes*
Tube MUST be
Citrate
3.5ml
filled to within
• Anti-Xa – 1 Tube*, Lupus Anticoagulant – 2
Tubes*
1st
White
the arrow
IMPORTANT:
When using a winged Blood Collection Set for these tests, a discard (non-additive
Blue
KFK 432) tube should be used. This is to ensure blood is not lost in the ‘dead space’ and the tube fills to 
within the arrow giving the correct blood to anticoagulant ratio.
MINIMUM VOLUME
MICROBIOLOGY REQUEST
*
Take to lab immediately
• Viral & Bacterial Serology
on ice
BIOCHEMISTRY REQUEST
C-Peptide*
Cryoglobulins**
Insulin*
• hCG
KFK 307
**
Take to lab immediately
Clotting
2nd
• Progesterone • Prolactin • All drugs (except
keep at 37 ºC
Accelerator
6ml
immunosuppressants)
Black
Red
TRANSFUSION REQUEST
• Cold
agglutinins**
HAEMATOLOGY REQUEST • HIT screen
IMMUNOLOGY REQUEST
• Immunology
BIOCHEMISTRY REQUEST
Clotting
KFK 305
Accelerator
• All general chemistry tests • All Endocrine tests;
3rd
*
Full Tube Required
and
(except those with special instructions - see 2nd & 7th)
4ml
Separation
Ochre
Ochre
Gel
• B12
*
• Folate
*
• Ferritin
*
KFK 320
BIOCHEMISTRY REQUEST
Li Hep.
4th
• Please use for unexpected raised potassium
3ml
Gel
Green
Yellow
BIOCHEMISTRY REQUEST
KFK 321
*
Discuss with duty
5th
• Aluminium
Li Heparin
Biochemist
6ml
• Specialised Chemistry Investigations
*
Green
Black
HAEMATOLOGY REQUEST
• FBC • Blood Film • ESR • G6PD
KFK 303
• Infectious mononucleosis • Malaria • HbA1c
6th
• Haemoglobinopathy Screen • Direct Antiglobulin Test
EDTA
4ml
TRANSFUSION REQUEST
Black
Lavender
• Crossmatch • Group & Screen
*
Take to lab immediately on ice
BIOCHEMISTRY REQUEST
**
Take to the lab at
ACTH*
Renin**
Ammonia*
KFK 303
room temperature
7th
• Immunosuppressant Drugs • PTH
EDTA
within 1 hour
4ml
MICROBIOLOGY REQUEST
Black
Lavender
Separate tubes required
• Microbiology PCR Investigations
for each test
BIOCHEMISTRY REQUEST
KFK 246
NaF/
Separate tubes required
8th
• Glucose
for each test
Oxlate
4ml
• Alcohol
Grey
Black
Sodium
KFK 262
Heparin
BIOCHEMISTRY REQUEST
9th
Trace
• Trace Elements (eg: Copper etc.)
6ml
Elements
Navy Blue
Black
IMPORTANT: Hold tube in place with
the
MIXING OF SPECIMEN MATERIAL
until filled to the required level
thumb
DO NOT SHAKE
Following blood collection, all tubes should be
VAUM02
VERSION 4
CHECK TUBE
June 2016
gently inverted 5-10 times (coagulation tubes
EXPIRY DATES.
4-5 times). Thorough mixing is necessary to
ensure adequate performance of the tube
CHECK
contents (additive) with the blood sample.
Greiner Bio-One Ltd.
RIGHT PATIENT,
Tel: 01453 825255.
A full inversion is when the air bubble moves
Fax: 01453 826266
RIGHT BLOOD.
from one end of the tube to the other.

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