Science Lab Template Affects On Enzyme Reaction Rate (Part A) Page 2

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Teacher sign off: _______________
*Now you have the green light to begin the experiment.
Analysis:
• Calculate the average foam production and overall rate of reaction for each temperature.
• Create a line graph to illustrate your data.
• Be sure to have a title and properly labeled axis.
• You will be graphing the rate of reaction versus temperature.
Rate of reaction(ml/min) = average amount of foam (ml)
reaction time (minutes)
Conclusion questions: (complete sentences please!)
1. At what temperature did the enzyme peroxidase work best? How do you know?
2. According to your data, discuss what the affects of temperature is on enzyme activity
(the rate of the reaction). In other words, how do changes in temperature affect the amount of foam
produced?
3. If you’ve ever used hydrogen peroxide as an antiseptic to treat a cut or scrape, you know that it
foams as soon as it touches an open wound. Based on your knowledge of peroxidase, how can you
explain this observation of the wound foaming up? (Refer to the above chemical reaction)
4. According to your graph, what temperature(s) might be considered dangerous to the enzymes in
your body? In other words, what temperature(s) denature the enzymes?
Sources of error:
• List the possible sources of error relative to your experimental (qualitative).
• What is causing the greatest error in this experiment?
Hypothesis conclusion: (write in paragraph form)
• Restate your hypothesis.
• Does your data support or reject your hypothesis?
• Explain your results (support your conclusions with data!)
Please follow the order
of your coversheet and
check the box on cover
sheet when completed.
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