Enzyme And Oligo Sequence Chart Page 3
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1
2
3SalI
GTCGACGTCAAAAGGCCATAGCGGCCGC
28
0
0
GCGTCGACGTCTTGGCCATAGCGGCCGCGG
30
10
50
ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA
32
10
75
ScaI
GAGTACTC
8
10
25
AAAAGTACTTTT
12
75
75
SmaI
CCCGGG
6
0
10
CCCCGGGG
8
0
10
CCCCCGGGGG
10
10
50
TCCCCCGGGGGA
12
>90
>90
SpeI
GACTAGTC
8
10
>90
GGACTAGTCC
10
10
>90
CGGACTAGTCCG
12
0
50
CTAGACTAGTCTAG
14
0
50
SphI
GGCATGCC
8
0
0
CATGCATGCATG
12
0
25
ACATGCATGCATGT
14
10
50
StuI
AAGGCCTT
8
>90
>90
GAAGGCCTTC
10
>90
>90
AAAAGGCCTTTT
12
>90
>90
XbaI
CTCTAGAG
8
0
0
GCTCTAGAGC
10
>90
>90
TGCTCTAGAGCA
12
75
>90
CTAGTCTAGACTAG
14
75
>90
XhoI
CCTCGAGG
8
0
0
CCCTCGAGGG
10
10
25
CCGCTCGAGCGG
12
10
75
XmaI
CCCCGGGG
8
0
0
CCCCCGGGGG
10
25
75
CCCCCCGGGGGG
12
50
>90
TCCCCCCGGGGGGA
14
>90
>90
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