Ptc Genetics Lab Student Worksheet Page 4
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PTC
Prelab Part 2
(Questions)
1.
What is DNA and what does it do?
2. What are chromosomes and how many copies do you have in each of your cells? Who did they come from?
3. How much DNA do you share with each of your parents? How much DNA do you share with your siblings?
4. What are some characteristics or properties of DNA?
Part I: Electrophoresis
Well
Sample Name
Volume
Materials
1
DNA M
10 μL
1 MiniOne Casting System
2
DNA D
10 μL
1 MiniOne Electrophoresis System
1 Agarose GreenGel in-a-Cup
3
DNA J
10 μL
6 DNA samples
4
DNA B1
10 μL
TBE running buffer in conical flask
1 micropipette (2-20 μL) & 6 pipette tips
5
DNA B2
10 μL
6
DNA G
10 μL
MiniOne Visual Instructions
How to Cast a Gel
1. Place the MiniOne Casting Stand on a
level surface and place gel trays in the two
cavities. The straight edge should be on the
Right side. Insert the comb into the slots at
the top of the casting stand with the 6 well
side facing down.
2. Partially peel the film of a GreenGel in-a-Cup and
00:20
microwave for 20 seconds. Allow to cool for 15 seconds.
DO NOT microwave more than 5 GelCups at a time.
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