Ptc Genetics Lab Student Worksheet Page 5
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10PTC
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3. Slowly pour the hot agarose solution into a
gel tray. Make sure there are no air bubbles
in the agarose solution. Let the agarose gel
solidify for 10 mins or until opaque. DO
NOT disturb the gel until time is up.
4. Carefully remove comb when gel is ready.
Remove gel tray with solidified gel from
Casting Stand and wipe off any access
agarose from the bottom of the tray
How to Load a Gel
1. Ensure the black viewing platform is in the
tank if it is not already installed and put the
gel (along with the gel tray) into the tank.
Make sure the wells are aligned with the
marks on the platform on the negative
end.
2. Measure 135 mL of TBE running buffer and
pour into one side of the tank to push out
the air, creating a nice even background
without air bubbles or air trapped for
imaging later.
3. Plug the power supply into the wall. Place
the tank into the carriage so the carbon
electrodes are touching the gold rivets and
the tank sits level with the carriage.
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