Secure urination facility before the collection (If
record the date and time of collection; sign
single-toilet room with a full-length privacy door)
statement; enter actual name of delivery service
(Parts 40.41 & 43).
transferring the specimen to laboratory (Part
- Secures any water sources or make them
40.73(a)(2)).
Ensure that all copies of the CCF are legible and
unavailable to employees (e.g., turn off water
inlet, tape handles to prevent opening faucets).
complete (Part 40.73(a)(3)).
Remove Copy 5 of the CCF and give it to the
- Ensures that the water in the toilet tank contains
bluing agent.
employee (Part 40.73(a)(4)).
Place specimen bottles and Copy 1 of CCF in plastic
- Ensures that soap, disinfectants, cleaning
agents, or other possible adulterants are not
bag and secure both pouches of plastic bag (Part
present.
40.73(a)(5)-(a)(6)).
Advise employee that he/she may leave the site
- Inspects the site to ensure that no foreign or
unauthorized substances are present.
(Part 40.73(a)(7)).
Recheck the urination facility, performing all steps as
- Tapes or otherwise securely shuts any movable
toilet tank or puts bluing agent in the tank.
was done prior to the collection to ensure the site’s
- Ensures that undetected access (e.g., through a
continued integrity.
door not in your view) is not possible.
- Secures areas and items (e.g., ledges, trash
receptacles, paper-towel holders, under-sink
areas, drop-down ceiling panels) that appear
suitable for concealing contaminants.
Direct employee to go into room used for urination
and instruct employee to:
- Provide at least 45 ml of urine.
- Not flush the toilet.
- Return specimen to the collector as soon as the
void is complete.
- Set a reasonable time limit for voiding (Part
40.63(d)(2)).
- Allow only the employee into the room used for
urination (40.41(d)(1)).
Check that the specimen:
- Contains at least 45 ml of urine. If not, follow shy
bladder procedure (Part 40.65(a)).
- Reads temperature strip within 4 minutes (Part
40.65(b)).
Mark appropriate box in Step 2 of CCF (Yes =
between 90 and 100 degrees).
Check specimen for signs of tampering (Part 40.65).
Check specimen for unusual color, foreign
objects/material, or other signs of tampering (odor).
Mark box in Step 2 of the CCF indicating a split
specimen collection (Part 40.71(b)(1)).
Pour at least 30 ml of urine into the primary
specimen bottle (Part 40.71(b)(2)).
Pour at least 15 ml of urine into the secondary
specimen bottle (Part 40.71(b)(2)).
Secure the lids or caps on the specimen bottles
(Part 40.71(b)(4)).
Place the tamper-evident seals on the specimen
bottles (Part 40.71(b)(5)).
- Dates the specimen bottle seals (Part
40.71(b)(6)).
- Ensures that the employee initials specimen
bottle seals (Part 40.71(b)(7)).
Direct employee to read and sign certification
statement on Copy 2, Step 5 of CCF and to provide
date of birth, printed name, day and evening contact
telephone numbers (Part 40.71(a)(1)).
Print collector name in Copy 1, Step 4 of CCF;
2