Dilutions And Solutions Worksheet

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Biology 324
Dilutions and Solutions
This worksheet will not be graded but similar questions may appear on your quiz.
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For lab work, you have to be able to make up solutions. Somehow, this process is often
perceived as ranging from difficult to impossible. This first lab will introduce you to the
calculations that are employed to make solutions. Don't be too disheartened if it seems
difficult. By the end of the quarter, you will be a walking abacus when it concerns
making solutions.
Most working laboratory solutions are expressed in terms of molar concentrations.
Question 1) What is a mole?
Note that if a solution is a % solution, it refers to the number of grams per total of 100
ml. For example, a 15% SDS solution is made by adding 15 grams of SDS to a total
volume of 100 ml.
Most chemicals give their gram/molecular weight on the bottle. From the
gram/molecular weight you should be able to figure out how much mass of a given
chemical you need to make up any given molar solution.
Example 1)
NaCl gram/molecular weight is 58.5 g.
To make 1 liter of a 1.0 molar NaCl solution, 58.5 g of NaCl is dissolved in a total of 1.0
liter of water.
Question 2) How much NaCl is needed to make a 100 ml of a 1.0 M solution?
Question 3) How much NaCl is needed to make 100 ml of a 5.0 M solution?
Dilution handout
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