Dilutions And Solutions Worksheet Page 6

This same approach can be used for mg/ml or percentages, e.g.,

(5) Solution wanted: 5 ml of 250 µg/ml BSA

Stock solution: 5 mg/ml BSA

Stock solution

final concentration

stock/final = ______X

Stock concentration

final volume

volume of stock to make 1X

To make 5 ml of 250 µg/ml BSA:

volume of 5 mg/ml BSA

volume of water

(6) Solution wanted: 150 ml of 0.5% SDS

Stock solution: 10% SDS

Stock solution

final concentration

stock/final = ______X

To make 150 ml of 0.5% SDS:

volume of 10% SDS

volume of water

Dilution handout

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