Quality Assurance Project Plan Including Sampling And Analysis Plan (Page 29 of 67) in pdf

Hunters Point Shipyard Parcel F ESTCP Demonstration Plan

Appendix A: Quality Assurance Project Plan

A.3.2.1

Plot Locations

GPS coordinates will be recorded in the field using a handheld GPS unit (Garmin Geko™ 201) with

WAAS-enabled accuracy of ±3 m. The exact GPS coordinates will be defined with the latitude and

longitude in terms of degrees and decimal minutes using WGS 84 datum. The dimensions of the plot are

on a similar scale to that of the unit’s accuracy, so only the center of each plot will be defined with GPS

coordinates. The locations of clam tube/SPMD deployments and core samples will be marked on a scale

map (with magnetic North identified) in relation to the center and corners of each plot. GPS coordinates

will be recorded for water column samples taken over the plot areas. The exact GPS coordinates will

define the latitude and longitude in terms of decimal degrees or degrees and decimal minutes.

A.3.2.2

Testing Material Deployment and Sampling of Environmental Media for Chemical

Analysis

A.3.2.2.1

SPMD Deployment and Retrieval

As shown in Table A-1, biomimetic SPMDs will be deployed in the sediment once before and twice after

AC treatment to simulate the in situ availability of PCBs to biota. The SPMDs used in this study will be

10 cm long and will contain the nonpolar lipid triolein. One SPMD will be vertically suspended inside

each clam tube onto two hooks mounted on the inner wall. Each end of the SPMD has a 3-cm-long loop.

These loops will be slipped onto the hooks that are 16 cm apart. The top loop of the SPMD will be

located 3 cm below the sediment surface. This design will allow the SPMD to be suspended and stretched

vertically, keeping it away from the clam tube wall. A total of 75 SPMDs will be deployed during the

entire project. Following field collection, the SPMDs will be sent to Stanford University for PCBs

analysis.

M. nasuta Tube Deployment and Retrieval

A.3.2.2.2

PCB bioaccumulation in test clams will be measured using particle-feeding M. nasuta clams native to San

Francisco Bay. The work shall use small organisms (6-g “whole clam with shell” weight, to reduce the

slow internal equilibration kinetics associated with larger organisms) of standard size (to minimize size-

related accumulation effects). These clams will be placed in mesh-covered PVC tubes sunk into the five

plots once before and twice after AC treatment at each of the five sampling locations, according to the

schedule shown in Table A-1. The clam tube will be 1.5 feet long and have a diameter of 6 inches. The

five clam tubes in each plot are considered experimental replicates. The six clams will be placed per clam

tube onto the sediment surface within the tube’s diameter and allowed to burrow. A total of 75 clam tubes

will be deployed during the entire project. After a 28-day exposure, the clams will be removed by

carefully scooping out the sediment in the clam tubes. The clams then will be separated from the

sediment, rinsed with site water, and placed in polyethylene containers. The organisms shall be depurated

in clean sediment for 24 hours and then in seawater for 48 hours at ambient temperatures before

sacrificing clams. Each clam will be shucked, and the resulting whole tissue will be placed in a separate

scintillation glass vial. At ERDC each set of six (or total number surviving) clams that came from a given

clam tube will be homogenized and split. Half will be shipped to BDO for archiving and while the other

half will be analyzed at ERDC. The in situ tests will conform to work of others employing planktonic

crustaceans, amphipods, shrimp, and oligochaete worms (e.g., Chappie and Burton, 1997).

A.3.2.2.3

Indigenous Amphipod Sample Collection

According to the schedule in Table A-1, five separate surface (0-2 cm) sediment samples shall be

collected at the sampling locations in each plot as shown in Figure A-5 and placed into a separate wide-

mouthed polyethylene jar with a vented lid. These jars shall be maintained at <18 °C in a cooler, and

Page A-

of A-67

Quality Assurance Project Plan Including Sampling And Analysis Plan Page 29

Related Articles

Related forms

Related Categories